NeoPlat Blast Mac OS

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Software Resources‎ > ‎MacQIIME‎ > ‎MacQIIME Installation‎ > ‎

Installing BLAST in OS X

These instructions apply to installing BLAST 2.2.22 in OS X, because this is the version that is supported and checked-for by QIIME.

Option 1: Installing BLAST within your user account

Follow these instructions to install BLAST in your home directory. Only do this if you are the only user on your computer. If the computer has multiple users, follow the instructions below for Option 2: Installing BLAST in a central location.
1.Download (but do not unzip) this file, and save in your Downloads folder:
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/2.2.22/blast-2.2.22-universal-macosx.tar.gz
2. Unzip it in the Terminal using tar:
cd ~/Downloads
tar -xvf blast-2.2.22-universal-macosx.tar.gz

This should create a new folder called blast-2.2.22
3. Move it somewhere permanent - in this example, we'll just put it in your home directory.
4. Add the ~/blast-2.2.22/bin folder to your PATH using the nano text editor:

nano ~/.profile

This will open the nano text editor, editing a file called .profile in your home directory. Here you can add this line, referencing the directory that contains the BLAST executables:
export PATH=/Users/YourName/blast-2.2.22/bin:${PATH}
where 'YourName' should be your username (or, '/Users/Yourname/' should be your home directory name). If you aren't sure what your home directory is, you can open a new terminal session and type 'cd ~' followed by 'pwd'. You can then save your .profile file in nano by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
Close your current Terminal session(s) and open a new one. Now, when you type this command:
it should state the location of the blastall executable file:
/Users/YourName/blast-2.2.22/bin/blastall


5. Make a ~/.ncbirc file that points to the BLAST data directory:
nano ~/.ncbirc

And put this text into the file:

where you should put in your username/home directory instead of 'YourName.' Save the .ncbirc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
You should now have a functioning copy of BLAST, ready to be called from QIIME or anything else.

Option 2: Installing BLAST in a central location

These instructions will put BLAST in a central location so any user can run BLAST searches. You need to have administrative privileges to do this (i.e., you need to be an sudoer). If you are the only user on your computer, Option 1 may be better.
1.Download (but do not unzip) this file, and save in your Downloads folder:
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/2.2.22/blast-2.2.22-universal-macosx.tar.gz
2. Unzip it in the Terminal using tar:
cd ~/Downloads
tar -xvf blast-2.2.22-universal-macosx.tar.gz

This should create a new folder called blast-2.2.22
3. Copy it somewhere permanent - in this example, we'll put it in the /opt directory at the root of the filesystem, so anyone can access the files.
(If the 'mkdir' command says that /opt already exists, this is okay.)

4. Add the /opt/blast-2.2.22/bin folder to your PATH using the nano text editor:

sudo nano /etc/bashrc

This will open the nano text editor, editing a file called /etc/bashrc. Here you can add this line, referencing the directory that contains the BLAST executables:
export PATH=/opt/blast-2.2.22/bin:${PATH}
You can then save the /etc/bashrc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
Close your current Terminal session and open a new one. Now, when you type this command:
it should state the location of the blastall executable file:
/opt/blast-2.2.22/bin/blastall


5. Make a ~/.ncbirc file that points to the BLAST data directory:
nano ~/.ncbirc

And put this text into the file:

Save the .ncbirc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x. EVERY USER needs to do this step in their own home directory. Any user running BLAST needs to have a ~/.ncbirc file.
You should now have a functioning copy of BLAST, ready to be called from QIIME or anything else.

TurboGenomics, which specializes in high-performance parallel and distributed bioinformatics software, has released its new package, TurboBLAST, for Mac OS X, at this week’s Macworld New York.

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TurboBLAST is an accelerated, parallel implementation of the unmodified BLAST homology search application distributed by the National Center for Biotechnology Information. TurboBLAST enables scientists to increase productivity by removing the computational bottleneck in the BLAST application.

TurboBLAST provides superlinear acceleration of the BLAST algorithm, dynamically distributing the workload across heterogeneous machine types and operating systems, including Mac OS X, Linux, Unix and Windows. It can run on dedicated clusters of workstations as well as idle desktop machines to maximize efficiency and throughput. TurboBLAST is the first product in the TurboTools suite, a group of parallel implementations of bioinformatics algorithms to be released by TurboGenomics.

“We’re thrilled to be able to showcase TurboBLAST’s superior performance with the power and elegance of Mac OS X on the G4,” said TurboGenomics CEO, Dr. Gregory Gardiner, in a statement. “TurboBLAST is the first of many parallel implementations of bioinformatics algorithms that will be developed by TurboGenomics for the Macintosh.”

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The innovative user interface provided by Mac OS X and the speed of the PowerPC G4 processor are an ideal combination for the scientist performing large BLAST calculations with TurboBLAST, he added. This release for Mac OS X leverages both the Aqua interface and the Darwin core to yield enhanced performance and usability, Gardiner said. Plus, the on-board gigabit Ethernet provides a performance boost by reducing the network overhead needed to transfer genomic databases.

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Computer farms are becoming widely accepted as attractive solutions for computationally intensive algorithms, Gardiner said. Distributed computing systems with parallel software implementations like TurboBLAST have proven to be cost-effective, flexible and fast compared to traditional supercomputing facilities, he added.